Flow Cytometry
The IRCM's Flow Cytometry Core Facility offers cell analysis and sorting services to IRCM researchers as well as to members of the Montreal and surrounding scientific communities. Our goal is to promote the use of flow cytometry as a tool for multiparametric analysis and to facilitate its use for a broad range of research fields. Immunophenotyping, apoptosis, cell cycle and DNA content, functional tests (calcium flow) and cell proliferation are among the techniques commonly used in cytometry.
Instrumentation
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Table top analyzers
BD Accuri C6, FACScalibur (488 and 635 lasers, 4 color detection), BD LSR Fortessa (488, UV, 405nm, 561nm and 635nm lasers, 18 color detection), Cyan (405nm, 488nm and 642nm lasers, 9 color detection), Sony SA 3800 (405nm, 488nm, 561nm and 642nm lasers).
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Cell sorting
MoFlo (Beckman Coulter) 3 lasers (488nm; UV; 642nm) with 8 color detection; BD Influx (BD Biosciences) (In NC3 confinement and under class 2 hood) with three lasers (488nm; 635nm; 405nm) and 9 color detection; BD FACSAria III (BD Biosciences) (Under class 1 hood) with five lasers (488nm; UV; 405nm; 561nm; 642nm) and 18 color detection.
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Other instrumentation
AutoMACS, automated device for cell separation using magnetic beads; extracellular flow analyzer XF24-3 (Seahorse Bioscience), a fully integrated instrument that simultaneously measures the two major energy pathways – aerobic respiration and glycolysis – in a convenient microplate format; ADVIA 120, a hematological analyzer on human and animal whole blood.