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Eric Massicotte Director |
Team : Julie Lord-Gagnon, Senior Research Assistant
The flow cytometry platform offers cell analysis and sorting services to IRCM researchers as well as to members of the Montreal and surrounding scientific communities. Our goal is to promote the use of flow cytometry as a tool for multiparametric analysis and to facilitate its use for a broad range of research areas. Immunophenotyping, apoptosis, cell cycle and DNA content, functional tests (calcium flow) and cell proliferation are among the techniques commonly used in cytometry.
Instrumentation
Table top analyzers
BD Accuri C6
FACScalibur (BD Biosciences) (488 and 635 lasers, 4 color detection)
BD LSR Fortessa (BD Biosciences) (488, UV, 405nm, 561nm and 635nm lasers, 18 color detection)
Cyan (Beckman Coulter) (405nm, 488nm and 642nm lasers, 9 color detection)
Sony SA 3800 (Sony Biosciences) (405nm, 488nm, 561nm and 642nm lasers)
Cell sorting
MoFlo (Beckman Coulter) 3 lasers (488nm; UV; 642nm) 8 color detection
BD Influx (BD Biosciences) (In NC3 confinement and under class 2 hood) with three lasers (488nm; 635nm; 405nm) and 9 color detection
BD FACSAria III (BD Biosciences) (Under class 1 hood) with five lasers (488nm; UV; 405nm; 561nm; 642nm) and 18 color detection
Services
The flow cytometry platform also offers services: data analysis, training and consultation.
Autre instrumentation
AutoMACS, automated device for cell separation using magnetic beads
Extracellular flow analyzer XF24-3 (Seahorse Bioscience), a fully integrated instrument that simultaneously measures the two major energy pathways – aerobic respiration and glycolysis – in a convenient microplate format
ADVIA 120, a hematological analyzer on human and animal whole blood
Contact the
Flow Cytometry services
110, ave des Pins West,
Montréal (Québec) Canada
H2W 1R7
514 987-5500
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