Microscopy and Imaging

The Microscopy and Imaging Core Facility is equipped with optical microscopes to acquire images or videos of various live or fixed samples. Several detection systems allow these acquisitions to be made. In addition, different software programs are used to process the acquired images and thus obtain digital data such as cell quantities, relative fluorescence intensities, surface measurements, displacement speeds, etc. The staff of the Microscopy and Imaging Core Facility rely on obtaining uniform images and videos whose quality allows their analysis by automatic computer programs. Finally, the Microscopy and Imaging Core Facility develops various customized analysis tools.

Team

Dominic Filion

Manager of the Core Facility

Phone:

514 987-5766

dominic.filion@ircm.qc.ca

Instrumentation

Confocal Microscopy

Confocal SP8, confocal LSM 700, confocal LSM 710, spinning disk Confocal (rotary disc confocal)
Interval Acquisition Microscopy

Timelapse CAY, Timelapse CHA
Fluorescence Epi Microscopy

DM6, DM6000, DM5500B, DM4000, DMRB
Histological Blade or Tissue Observation

Straight Microscope DM4000B – Osteomeasure, straight Axiophot Microscope, dissecting microscopeMZ12, MZ16FA microscope
Observation of samples in Petri dishes or multi-well plates

Axiovert S100TV inverted microscope, DMIRE inverted microscope
Highly Specialized Techniques

Optical tomograph, laser dissecting microscope, MetaXpress high-speed imaging
Customized analysis

The analysis station is a high-performance computer where several image, video and volumetric data files analysis software are installed, sometimes combined with time data. Bitplane's Imaris software is installed on this 3D image and video processing station on which is installed. This software processes particularly uniquely large files (sometimes several GB) and allows the analysis of otherwise untreatable files. The responsible person, Dominic Filion, holds a full personal programmer license to this software.