Microscopy and Imaging
The Microscopy and Imaging Core Facility is equipped with optical microscopes to acquire images or videos of various live or fixed samples. Several detection systems allow these acquisitions to be made. In addition, different software programs are used to process the acquired images and thus obtain digital data such as cell quantities, relative fluorescence intensities, surface measurements, displacement speeds, etc. The staff of the Microscopy and Imaging Core Facility rely on obtaining uniform images and videos whose quality allows their analysis by automatic computer programs. Finally, the Microscopy and Imaging Core Facility develops various customized analysis tools.
Instrumentation
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Confocal Microscopy
Confocal SP8, confocal LSM 700, confocal LSM 710, spinning disk Confocal (rotary disc confocal)
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Interval Acquisition Microscopy
Timelapse CAY, Timelapse CHA
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Fluorescence Epi Microscopy
DM6, DM6000, DM5500B, DM4000, DMRB
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Histological Blade or Tissue Observation
Straight Microscope DM4000B – Osteomeasure, straight Axiophot Microscope, dissecting microscopeMZ12, MZ16FA microscope
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Observation of samples in Petri dishes or multi-well plates
Axiovert S100TV inverted microscope, DMIRE inverted microscope
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Highly Specialized Techniques
Optical tomograph, laser dissecting microscope, MetaXpress high-speed imaging
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Customized analysis
The analysis station is a high-performance computer where several image, video and volumetric data files analysis software are installed, sometimes combined with time data. Bitplane's Imaris software is installed on this 3D image and video processing station on which is installed. This software processes particularly uniquely large files (sometimes several GB) and allows the analysis of otherwise untreatable files. The responsible person, Dominic Filion, holds a full personal programmer license to this software.