Molecular Biology and Functional Genomics
The Molecular Biology and Functional Genomics Core Facility offers a range of services combining advice and access to cutting-edge techniques to meet gene analysis needs.
Offered services:
- Sanger sequencing for sequence identification and mutation detection,
- Fluorescence fragment genotyping for transgene and SNP detection
- High-throughput sequencing, on Illumina Hi-Seq 2500 sequencer (whole genome, exome capture; ChIP Seq, METHYL Seq, FAIRE Seq, ATAC Seq; RNA Seq (specific strand), microRNA, RIP Seq)
- Quality control of nucleic acid samples
- Dedicated instrumentation
Instrumentation and services
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Sequencing by the Sanger method
Sequencing of plasmids, cosmids, BACs and PCR products, for readings from 250 bp to 1,000 bp; use of BigDyeV3.1 chemistry (ThermoFisher); universal primers available. Instrument: 3500xl Genetic Analyzer - 24 capillaries - 50 cm (ThermoFisher)
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Genotyping
Analysis of fluorescent PCR fragments (between 150 bp and 1,200 bp) by capillary electrophoresis; Instrument: 3500xl Genetic Analyzer - 24 capillaries - 50 cm (ThermoFisher). Presence-absence of transgenes; Instrument: Q-PCR ViiA 7 (ThermoFisher).
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High flow sequencing
Genome study (whole genome, exome capture; Methyl-Seq; ChIP-Seq, FAIRE-Seq, ATAC-Seq), transcriptome study (RNA-Seq specific strand: mRNA enrichment or rRNA depletion; RIP-Seq; Small RNA), possible protocol development on request. Instrument: HiSeq 2500 from Illumina.
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Quality control of nucleic acid samples
RNA: determination of quantity and integrity (RNA Integrity Number - RIN); DNA: determination of size and quantification of PCR fragments or fragments resulting from shearing, validation of libraries. Instrument: 2100 Bioanalyzer (Agilent).
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Instrumentation available: Q-PCRs
Two instruments: ThermoFisher ViiA-7 - with drawers for 384 plates, 96 wells and microfluidic cards with 384 reactions.
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Instrumentation available: Ultra-Sonicator
Instrument: Covaris E220; Adaptive Focused Acoustic (AFA) technology for constant and reproducible fragmentation; chomatin or DNA sonication with 6x16 mm microtubes (50 or 130µl volumes) or 12x12 mm millitubes tubes (1ml volume).
Additional information on the Core Facility
Download the presentation sheet of the Molecular Biology and Functional Genomics Core Facility to learn about all the services and equipment available.